The cycle of life of a handful of different animals is captured on film in a whole new way in this documentary. Using special motion-control photography equipment, Genesis allows filmgoers to view animal behaviors which are too small, too slow, or too difficult to normally be seen with the naked eye, including a chick hatching its way out from inside an egg, jellyfish drying into nothing under the heat of the sun, or a snake slowly swallowing prey bigger than itself. This footage is accompanied by narration from Sotigui Kouyate, who uses simple props and easily understandable analogies to explain the science behind what its shown on screen. Genesis was directed by Claude Nuridsany and Marie Perennou, who previously created another acclaimed scientific documentary, Microcosmos.

This episode covers the nature of how life may have developed on Earth and the possibility of life on other planets. Tyson begins by explaining how the human development of writing systems enabled the transfer of information through generations, describing how Princess Enheduanna ca. 2280 BCE would be one of the first to sign her name to her works, and how Gilgamesh collected stories, including that of Utnapishtim documenting a great flood comparable to the story of Noah's Ark. Tyson explains how DNA similarly records information to propagate life, and postulates theories of how DNA originated on Earth, including evolution from a shallow tide pool, or from the ejecta of meteor collisions from other planets. In the latter case, Tyson explains how comparing the composition of the Nakhla meteorite in 1911 to results collected by the Viking program demonstrated that material from Mars could transit to Earth, and the ability of some microbes to survive the harsh conditions of space. With the motions of solar systems through the galaxy over billions of years, life could conceivably propagate from planet to planet in the same manner. Tyson then moves on to consider if life on other planets could exist. He explains how Project Diana performed in the 1960s showed that radio waves are able to travel in space, and that all of humanity's broadcast signals continue to radiate into space from our planet. Tyson notes that projects have since looked for similar signals potentially emanating from other solar systems. Tyson then explains that the development and lifespan of extraterrestrial civilizations must be considered for such detection to be realized. He notes that civilizations can be wiped out by cosmic events like supernovae, natural disasters such as the Toba disaster, or even self-destruct through war or other means, making probability estimates difficult. Tyson describes how elliptical galaxies, in which some of the oldest red dwarf stars exist, would offer the best chance of finding established civilizations. Tyson concludes that human intelligence properly applied should allow our species to avoid such disasters and enable us to migrate beyond the Earth before the Sun's eventual transformation into a red giant.

Ideas about time and space are explored in the changes that constellations undergo over time, the redshift and blue shift measured in interstellar objects, time dilation in Albert Einstein's theory of relativity, the designs of both Leonardo da Vinci and spacecraft that could travel near light speed, time travel and its hypothetical effects on human history, the origins of the Solar System, the history of life, and the immensity of space. In Cosmos Update, the idea of faster-than-light travel by wormholes (researched by Kip Thorne and shown in Sagan’s novel Contact) is discussed.

On November 25th, 1915, Einstein published his greatest work: General Relativity. The theory transformed our understanding of nature’s laws and the entire history of the cosmos, reaching back to the origin of time itself. Now, in celebration of the 100th anniversary of Einstein’s achievement, discover the inside story of Einstein’s masterpiece". The story begins with the intuitive thought experiments that set Einstein off on his quest and traces the revolution in cosmology that is still playing out in today’s labs and observatories. Discover the simple but powerful ideas at the heart of relativity, illuminating the theory—and Einstein’s brilliance—as never before. From the first spark of an idea to the discovery of the expanding universe, the Big Bang, black holes, and dark energy, NOVA uncovers the inspired insights and brilliant breakthroughs of “the perfect theory.”

Sagan discusses the story of the Heike crab and artificial selection of crabs resembling samurai warriors, as an opening into a larger discussion of evolution through natural selection (and the pitfalls of intelligent design). Among the topics are the development of life on the Cosmic Calendar and the Cambrian explosion; the function of DNA in growth; genetic replication, repairs, and mutation; the common biochemistry of terrestrial organisms; the creation of the molecules of life in the Miller-Urey experiment; and speculation on alien life (such as life in Jupiter's clouds). In the Cosmos Update ten years later, Sagan remarks on RNA also controlling chemical reactions and reproducing itself and the different roles of comets (potentially carrying organic molecules or causing the Cretaceous–Paleogene extinction event).

As man moves to colonize the cosmos, the realities of sexual relationships and reproduction need to be addressed. Probe the physiological, psychological and cultural challenges of sex in space.