Beginning with the separation of the fuzzy thinking and pious fraud of astrology from the careful observations of astronomy, Sagan follows the development of astronomical observation. Beginning with constellations and ceremonial calendars (such as those of the Anasazi), the story moves to the debate between Earth and Sun-centered models: Ptolemy and the geocentric worldview, Copernicus' theory, the data-gathering of Tycho Brahe, and the achievements of Johannes Kepler (Kepler's laws of planetary motion and the first science-fiction novel).

Sagan discusses comets and asteroids as planetary impactors, giving recent examples of the Tunguska event and a lunar impact described by Canterbury monks in 1178. It moves to a description of the environment of Venus, from the previous fantastic theories of people such as Immanuel Velikovsky to the information gained by the Venera landers and its implications for Earth's greenhouse effect. The Cosmos Update highlights the connection to global warming.

The journeys of the Voyager probes is put in the context of the Netherlands in the seventeenth century, with a centuries-long tradition of sailing ship explorers, and its contemporary thinkers (such as Constantijn Huygens and his son Christian). Their discoveries are compared to the Voyager probes' discoveries among the Jovian and Saturn systems. In Cosmos Update, image processing reconstructs Voyager’s worlds and Voyager’s last portrait of the Solar System as it leaves is shown.

Questions are raised about the search for intelligent life beyond the Earth, with UFOs and other close encounters refuted in favor of communications through SETI and radio telescope such as the Arecibo Observatory. The probability of technically advanced civilizations existing elsewhere in the Milky Way is interpreted using the Drake equation and a future hypothetical Encyclopedia Galactica is discussed as a repository of information about other worlds in the galaxy. The Cosmos Update notes that there have been fewer sightings of UFOs and more stories of abductions, while mentioning the META scanning the skies for signals.

Until World War II, Latino immigration to the United States was overwhelmingly Mexican-American. Now three new waves bring large-scale immigration from Puerto Rico, Cuba, and the Dominican Republic. As the Puerto Rican government implements a historic overhaul over a million Puerto Ricans are encouraged to leave for the US mainland, to alleviate the economic pressure. A young Juanita Sanabria arrives in New York, works hard in the garment district, but encounters hostility and discrimination. Ethnic tensions explode in youth gang warfare depicted in films like West Side Story, etching the stereotype of the knife wielding Puerto Rican in the American consciousness.

In the 1960s and 1970s a generation of Mexican Americans, frustrated by persistent discrimination and poverty, find a new way forward, through social action and the building of a new "Chicano" identity. The movement is ignited when farm workers in the fields of California, led by Cesar Chavez and Dolores Huerta, march on Sacramento for equal pay and humane working conditions. Through plays, poetry and film, Luis Valdez and activist Corky Gonzalez create a new appreciation of the long history of Mexicans in the South West and the Mestizo roots of Mexican Americans. In Los Angeles, Sal Castro, a schoolteacher, leads the largest high school student walkout in American history, demanding that Chicano students be given the same educational opportunities as Anglos. In Texas, activists such as Jose Angel Gutierrez, create a new political party and change the rules of the electoral game. By the end of the 1970s Chicanos activism and identity have transformed what it means to be an American. Chicano and Latino studies are incorporated into school curriculum; Latinos are included in the political process.